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The Encapsulation of Cell-free Transcription and Translation Machinery in Vesicles for the Construction of Cellular Mimics

DOI :

10.3791/51304-v

10:28 min

October 21st, 2013

October 21st, 2013

14,657 Views

1Centre for Integrative Biology, University of Trento

Herein we describe simple methods for the preparation of vesicles, the encapsulation of transcription and translation machinery, and the monitoring of protein production. The resulting cell-free systems can be used as a starting point from which to build increasingly complex cellular mimics.

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Cell free Transcription

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