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Juxtasomal Biocytin Labeling to Study the Structure-function Relationship of Individual Cortical Neurons

DOI :

10.3791/51359-v

February 25th, 2014

February 25th, 2014

13,437 Views

1Department of Integrative Neurophysiology, Center for Neurogenomics and Cognitive Research, Neuroscience Campus Amsterdam, VU University Amsterdam

To understand the structure of neuronal networks, functional and morphological characterization of individual neurons is a necessity. Here, we demonstrate juxtasomal biocytin labeling, which allows electrophysiological recordings in the extracellular configuration, yet maintaining the ability to intracellularly label the neuron for post hoc reconstruction of dendritic and axonal architecture.

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Juxtasomal Biocytin Labeling

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