JoVE Logo
Faculty Resource Center

Sign In

Juxtasomal Biocytin Labeling to Study the Structure-function Relationship of Individual Cortical Neurons

DOI :

10.3791/51359-v

February 25th, 2014

February 25th, 2014

13,433 Views

1Department of Integrative Neurophysiology, Center for Neurogenomics and Cognitive Research, Neuroscience Campus Amsterdam, VU University Amsterdam

To understand the structure of neuronal networks, functional and morphological characterization of individual neurons is a necessity. Here, we demonstrate juxtasomal biocytin labeling, which allows electrophysiological recordings in the extracellular configuration, yet maintaining the ability to intracellularly label the neuron for post hoc reconstruction of dendritic and axonal architecture.

Tags

Juxtasomal Biocytin Labeling

-- Views

Related Videos

article

Laser Capture Microdissection of Drosophila Peripheral Neurons

article

Nucleofection and Primary Culture of Embryonic Mouse Hippocampal and Cortical Neurons

article

Bilaminar Co-culture of Primary Rat Cortical Neurons and Glia

article

DiI-Labeling of DRG Neurons to Study Axonal Branching in a Whole Mount Preparation of Mouse Embryonic Spinal Cord

article

In Vivo Two-photon Imaging Of Experience-dependent Molecular Changes In Cortical Neurons

article

A Method for High Fidelity Optogenetic Control of Individual Pyramidal Neurons In vivo

article

Genetic Manipulation of Cerebellar Granule Neurons In Vitro and In Vivo to Study Neuronal Morphology and Migration

article

Differential Labeling of Cell-surface and Internalized Proteins after Antibody Feeding of Live Cultured Neurons

article

Laser Capture Microdissection - A Demonstration of the Isolation of Individual Dopamine Neurons and the Entire Ventral Tegmental Area

article

Immunohistochemistry and Multiple Labeling with Antibodies from the Same Host Species to Study Adult Hippocampal Neurogenesis

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved