Identification of Protein Interaction Partners in Mammalian Cells Using SILAC-immunoprecipitation Quantitative Proteomics
31.4K Views
•
12:53 min
•
July 6th, 2014
DOI :
July 6th, 2014
•Chapters in this video
0:05
Title
1:49
Harvesting of SILAC-labeled Cell Lysates
3:39
Binding Lysate to Anti-GFP Beads
4:45
Preparation of Samples for MS Analysis
6:11
Data Analysis I: Understanding the Results and Removal of Low-confidence Identifications
7:36
Data Analysis II: Selecting High Confidence Interactions for Further Study
8:58
Data Analysis III: Merging Replica Datasets
10:59
Results: Identification of Translation Initiation Factor – Binding Proteins
12:22
Conclusion
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