Rapid Identification of Gram Negative Bacteria from Blood Culture Broth Using MALDI-TOF Mass Spectrometry

24.2K Views

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13:29 min

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May 28th, 2014

DOI :

10.3791/51663-v

May 28th, 2014

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Timothy J. Gray¹, Lee Thomas¹, Tom Olma¹, David H. Mitchell¹, Jon R. Iredell¹^,²^,³, Sharon C. A. Chen¹^,³

¹Centre for Infectious Diseases and Microbiology Laboratory Services, Institute of Clinical Pathology and Medical Research, Westmead Hospital, ²Centre for Research Excellence in Critical Infection, Westmead Millennium Institute, Westmead Hospital, ³Sydney Emerging Infectious Diseases Institute, University of Sydney, Westmead Hospital

Summary

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Rapid Identification

Gram Negative Bacteria

Blood Culture Broth

MALDI TOF Mass Spectrometry

Clinical Microbiology

Bacterial Identification

Bloodstream Infection

Sub culture

Solid Agar

MALDI TOF MS

Blood Culture Specimens

Interfering Resins

Proteins

Hemoglobin

Bacterial Concentration

Bacterial Purification

Bacterial Extraction

Chapters in this video

0:05

Title

1:20

Preparation of Bacterial Inoculate from Blood Culture Broth

5:46

Preparation of MALDI-TOF Target Plate

6:34

MALDI-TOF MS Spectra Acquisition

8:42

Post Analysis-Interpretation of MALDI-TOF MS Scores

10:18

Representative MALDI-TOF Analysis of Gram Negative Bacteria from Blood Cultures

12:35

Conclusion

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