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JoVE Journal

Bioengineering

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3D Orbital Tracking in a Modified Two-photon Microscope: An Application to the Tracking of Intracellular Vesicles

10.1K Views

11:28 min

October 1st, 2014

October 1st, 2014

10,112 Views

0:05

Title

1:50

Sample Preparation

3:19

Microscope Configurations

5:05

Imaging

7:21

Trajectory Analysis

8:40

Results: Fast 3D Single Particle Tracking of an Isolated Lysosome Moving Inside the Cell After the Endosome Maturation Process

10:36

Conclusion

In this video protocol we track - at high speed and in three dimensions - fluorescently labeled lysosomes within living cells, using the orbital tracking method in a modified two-photon microscope.

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