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Combining Magnetic Sorting of Mother Cells and Fluctuation Tests to Analyze Genome Instability During Mitotic Cell Aging in Saccharomyces cerevisiae

DOI :

10.3791/51850-v

11:08 min

October 16th, 2014

October 16th, 2014

12,243 Views

1Department of Biological Sciences, Rensselaer Polytechnic Institute

Mutation rates in young Saccharomyces cerevisiae cells measured through fluctuation tests are used to predict mutation frequencies for mother cells of different replicative ages. Magnetic sorting and flow cytometry are then used to measure actual mutation frequencies and age of mother cells to identify any deviations from predicted mutation frequencies.

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Magnetic Sorting

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