Identify Potential Genetic Interactions for Screening
2:35
Design the Cloning Strategy to Clone SIR2 into the pRS315 Vector
5:46
Transform the Vector into atg1â–³ and Wild Type Yeast Strains
7:32
Determine the Chronological Life Span to Test for Shortened CLS Phenotype Suppression
8:13
Results: Chronological Lifespan of atg1â–³+pRS315-SIR2
10:07
Conclusion
Transcript
Aging is characterized by the time-dependent deterioration of multiple cellular processes that ultimately increases the probability of organismal death. Within a population, this process is variable and a number of genetic and environmental factor
Sign in or start your free trial to access this content
Here is a protocol to identify genetic interactions through an increased copy number suppressor screen in Saccharomyces cerevisiae. This method allows researchers to identify, clone, and test suppressors in short-lived yeast mutants. We test the effect of the copy number increase of SIR2 on lifespan in an autophagy null mutant.