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A Method for Screening and Validation of Resistant Mutations Against Kinase Inhibitors

DOI :

10.3791/51984-v

December 7th, 2014

December 7th, 2014

14,719 Views

1Divisions of Experimental Hematology and Cancer Pathology, Cancer Blood Disease Institute, Cincinnati Children's Hospital Medical Center

Emergence of genetic resistance against kinase inhibitor therapy poses significant challenge for effective cancer therapy. Identification and characterization of resistant mutations against a newly developed drug helps in better clinical management and next generation drug design. Here, we describe our protocol for in vitro screening and validation of resistant mutations.

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Screening

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