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Pooled shRNA Library Screening to Identify Factors that Modulate a Drug Resistance Phenotype

DOI :

10.3791/63383-v

June 17th, 2022

June 17th, 2022

3,021 Views

1Department of Clinical Haematology, Christian Medical College, 2Department of Biotechnology, Thiruvalluvar University, 3Centre for Stem Cell Research, Christian Medical College

High-throughput RNA interference (RNAi) screening using a pool of lentiviral shRNAs can be a tool to detect therapeutically relevant synthetic lethal targets in malignancies. We provide a pooled shRNA screening approach to investigate the epigenetic effectors in acute myeloid leukemia (AML).

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Keywords Pooled ShRNA Library

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