Porous Silicon Microparticles for Delivery of siRNA Therapeutics

10.9K Views

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08:31 min

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January 15th, 2015

DOI :

10.3791/52075-v

January 15th, 2015

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Jianliang Shen*¹^,², Xiaoyan Wu*¹^,³, Yeonju Lee¹, Joy Wolfram¹^,⁴, Zhizhou Yang¹, Zong-Wan Mao², Mauro Ferrari¹^,⁵, Haifa Shen¹^,⁶

¹Department of Nanomedicine, Houston Methodist Research Institute, ²MOE Key Laboratory of Bioinorganic and Synthetic Chemistry, School of Chemistry and Chemical Engineering, Sun Yat-sen University, ³Pediatrics Department of Union Hospital, Huazhong University of Science and Technology, ⁴CAS Key Laboratory for Biomedical Effects of Nanomaterials & Nanosafety, National Center for Nanoscience & Technology of China, ⁵Department of Medicine, Weill Cornell Medical College, ⁶Department of Cell and Developmental Biology, Weill Cornell Medical College

Summary

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Keywords Porous Silicon Microparticles

SiRNA Delivery

Polyethylenimine

Arginine

Nanoparticle Formation

Gene Silencing

Biocompatible

Cytotoxicity

Transfection Efficiency

Chapters in this video

0:05

Title

0:58

PCPS Particle Preparation

3:46

PCPS Particle Characterization

4:33

Loading of siRNA into PCPS Particles and Optimization of siRNA/PCPS Particle Ratio

5:31

Release of siRNA from PCPS Particles

6:16

Results: Characterization of Porous Silicon Microparticles for siRNA Delivery

8:02

Conclusion

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