JoVE Logo
Faculty Resource Center

Sign In

Nanoparticle-mediated siRNA Gene-silencing in Adult Zebrafish Heart

DOI :

10.3791/58054-v

July 29th, 2018

July 29th, 2018

7,887 Views

1Beijing Key Laboratory of Cardiometabolic Molecular Medicine, State Key Laboratory of Natural and Biomimetic Drugs, Institute of Molecular Medicine, Peking University, 2Department of Biomedical Engineering, College of Engineering, Peking University

It remains a major challenge to develop conditional gene-knockout or effective gene-knockdown in adult zebrafish organs. Here we report a protocol for performing nanoparticle-mediated siRNA gene-silencing in adult zebrafish heart, thus providing a new loss-of-function method for studying adult organs in zebrafish and other model organisms.

Tags

Nanoparticle mediated

-- Views

Related Videos

article

Kidney Regeneration in Adult Zebrafish by Gentamicin Induced Injury

article

Large-scale Zebrafish Embryonic Heart Dissection for Transcriptional Analysis

article

Analysis of Zebrafish Larvae Skeletal Muscle Integrity with Evans Blue Dye

article

Isolation and Culture of Adult Zebrafish Brain-derived Neurospheres

article

Isolation and Characterization of Single Cells from Zebrafish Embryos

article

Biosensing Motor Neuron Membrane Potential in Live Zebrafish Embryos

article

Culture of Adult Transgenic Zebrafish Retinal Explants for Live-cell Imaging by Multiphoton Microscopy

article

Dissection and Immunofluorescent Staining of Mushroom Body and Photoreceptor Neurons in Adult Drosophila melanogaster Brains

article

The Isolation and Culture of Primary Epicardial Cells Derived from Human Adult and Fetal Heart Specimens

article

Whole-mount Confocal Microscopy for Adult Ear Skin: A Model System to Study Neuro-vascular Branching Morphogenesis and Immune Cell Distribution

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved