JoVE Logo
Faculty Resource Center

Sign In

Isolation of Mitochondria from Mouse Skeletal Muscle for Respirometric Assays

DOI :

10.3791/63336-v

February 10th, 2022

February 10th, 2022

4,356 Views

1Centro Andaluz de Biología del Desarrollo, CSIC-UPO-JA, Universidad Pablo de Olavide, 2CIBERER, Instituto de Salud Carlos III

Here, we describe a detailed method for mitochondria isolation from mouse skeletal muscle and the subsequent analysis of respiration by Oxygen Consumption Rate (OCR) using microplate-based respirometric assays. This pipeline can be applied to study the effects of multiple environmental or genetic interventions on mitochondrial metabolism.

Tags

Keywords Mitochondria Isolation

-- Views

Related Videos

article

Adult and Embryonic Skeletal Muscle Microexplant Culture and Isolation of Skeletal Muscle Stem Cells

article

Mitochondrial Isolation from Skeletal Muscle

article

Purification of Progenitors from Skeletal Muscle

article

Laser-inflicted Injury of Zebrafish Embryonic Skeletal Muscle

article

Isolation and Culture of Individual Myofibers and their Satellite Cells from Adult Skeletal Muscle

article

Analysis of Skeletal Muscle Defects in Larval Zebrafish by Birefringence and Touch-evoke Escape Response Assays

article

Isolation of Blood-vessel-derived Multipotent Precursors from Human Skeletal Muscle

article

Isolation and Functional Analysis of Mitochondria from Cultured Cells and Mouse Tissue

article

Isolation of Mitochondria from Minimal Quantities of Mouse Skeletal Muscle for High Throughput Microplate Respiratory Measurements

article

Isolation of Intact Mitochondria from Skeletal Muscle by Differential Centrifugation for High-resolution Respirometry Measurements

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved