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Mitochondrial Isolation from Skeletal Muscle

DOI :

10.3791/2452-v

March 30th, 2011

March 30th, 2011

28,363 Views

1Department of Physiology, University of Kentucky

This protocol describes a procedure to study the respiration of mitochondria isolated from skeletal muscles. This method was adapted from Scorrano et al. (2007). The mitochondrial isolation procedure requires about 2 hours. The mitochondrial respiration can be completed in about 1 hour.

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Mitochondrial Isolation

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