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Derivation of T Cells In Vitro from Mouse Embryonic Stem Cells

DOI :

10.3791/52119-v

October 14th, 2014

October 14th, 2014

10,759 Views

1Department of Biological Sciences, Hunter College and Graduate Center, City University of New York, 2Sunnybrook Research Institute, Department of Immunology, University of Toronto

Mouse embryonic stem cells can be differentiated to T cells in vitro using the OP9-DL1 co-culture system. Success in this procedure requires careful attention to reagent/cell maintenance, and key technique sensitive steps. Here we discuss these critical parameters and provide a detailed protocol to encourage adoption of this technology.

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T Cells

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