JoVE Logo

Sign In

Derivation of T Cells In Vitro from Mouse Embryonic Stem Cells

10.9K Views

08:27 min

October 14th, 2014

DOI :

10.3791/52119-v

October 14th, 2014


Explore More Videos

Keywords Mouse Embryonic Stem Cells

Chapters in this video

0:05

Title

1:38

Days 0 - 5: Initiation of Coculture and Trypsin-mediated Passage with Preplating

3:55

Day 8: Hematopoietic Progenitor Cell (HPC) Collection

5:14

Day 10 - 12: Media Change and No Trypsin Passage

6:30

Results: Representative Flow Cytometry Analyses at Specific time Points During mESC Differentiation In Vitro

7:38

Conclusion

Related Videos

article

22:06

Derivation of Hematopoietic Stem Cells from Murine Embryonic Stem Cells

13.3K Views

article

10:14

Retroviral Transduction of T-cell Receptors in Mouse T-cells

34.8K Views

article

08:56

Characterization of Thymic Settling Progenitors in the Mouse Embryo Using In Vivo and In Vitro Assays

8.2K Views

article

11:56

Derivation of Human Embryonic Stem Cells by Immunosurgery

33.8K Views

article

10:44

A Three-dimensional Thymic Culture System to Generate Murine Induced Pluripotent Stem Cell-derived Tumor Antigen-specific Thymic Emigrants

7.1K Views

article

12:59

Derivation of Stem Cell Lines from Mouse Preimplantation Embryos

8.3K Views

article

07:12

Mouse Naïve CD4+ T Cell Isolation and In vitro Differentiation into T Cell Subsets

51.8K Views

article

10:19

Derivation of Mouse Trophoblast Stem Cells from Blastocysts

18.1K Views

article

11:56

Generation of Mice Derived from Induced Pluripotent Stem Cells

21.4K Views

article

07:46

In Vitro Differentiation of Naive CD4+ T Cells into Pathogenic Th17 Cells in Mouse

2.5K Views

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2025 MyJoVE Corporation. All rights reserved