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An Ex vivo Model of an Oligodendrocyte-directed T-Cell Attack in Acute Brain Slices

DOI :

10.3791/52205-v

February 5th, 2015

February 5th, 2015

6,763 Views

1Department of Neurology, University of Münster, 2Germany and Interdisciplinary Center for Clinical Research (IZKF) Münster, 3Institute of Physiology I - Neuropathophysiology I, University of Münster

To address mechanisms of demyelination and neuronal apoptosis in cortical lesions of inflammatory demyelinating disorders, different animal models are used. We here describe an ex vivo approach by using oligodendrocyte-specific CD8+ T-cells on brain slices, resulting in oligodendroglial and neuronal death. Potential applications and limitations of the model are discussed.

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Ex Vivo Model

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