JoVE Logo
Faculty Resource Center

Sign In

Automated Measurement of Pulmonary Emphysema and Small Airway Remodeling in Cigarette Smoke-exposed Mice

DOI :

10.3791/52236-v

January 16th, 2015

January 16th, 2015

12,962 Views

1Division of Pulmonary and Critical Care Medicine, Brigham and Women's Hospital - Harvard Medical School, 2Department of Respiratory Medicine, University of Cambridge - Addenbrooke's Hospital, 3Lung Transplant Program, Brigham and Women's Hospital - Harvard Medical School, 4COPD and IPF Programs, Lovelace Respiratory Research Institute

The goal of this protocol is to provide automated methods to quantify chronic lung pathologies in a murine model of COPD. The protocol includes exposing mice to cigarette smoke (CS), measuring pulmonary function, inflating the lungs, and using morphometry methods to measure emphysema and small airway remodeling in mice.

Tags

Automated Measurement

-- Views

Related Videos

article

Isolation of Mouse Respiratory Epithelial Cells and Exposure to Experimental Cigarette Smoke at Air Liquid Interface

article

Protocol for Long Duration Whole Body Hyperthermia in Mice

article

In vivo Measurement of the Mouse Pulmonary Endothelial Surface Layer

article

Echocardiographic Assessment of the Right Heart in Mice

article

Automated Measurement of Microcirculatory Blood Flow Velocity in Pulmonary Metastases of Rats

article

An Improved Method for Rapid Intubation of the Trachea in Mice

article

A Model of Cardiac Remodeling Through Constriction of the Abdominal Aorta in Rats

article

Disposable Dosators for Pulmonary Insufflation of Therapeutic Agents to Small Animals

article

Comparing the Effects of Electronic Cigarette Vapor and Cigarette Smoke in a Novel In Vivo Exposure System

article

Lung Fixation under Constant Pressure for Evaluation of Emphysema in Mice

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved