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Lung Fixation under Constant Pressure for Evaluation of Emphysema in Mice

DOI :

10.3791/58197-v

September 26th, 2019

September 26th, 2019

8,287 Views

1Department of Respiratory Medicine, Juntendo University Graduate School of Medicine, Juntendo University, 2Pharmaceutical Planning Group, Otsuka Pharmaceutical Co., Ltd.

Presented here is a useful protocol for lung fixation that creates a stable condition for histological evaluate of lung specimens from a mouse model of emphysema. The main advantage of this model is that it can fix many lungs with the same constant pressure without lung collapse or deflation.

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