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A 3D Cartographic Description of the Cell by Cryo Soft X-ray Tomography

DOI :

10.3791/62190-v

8:47 min

March 15th, 2021

March 15th, 2021

3,177 Views

1MISTRAL beamline, Alba Light Source, Cerdanyola del Valles, Spain, 2Beamline B24, Diamond Light Source, Harwell Science and Innovation Campus, Didcot, UK

Here, a protocol describing the sample preparation and data collection steps required in cryo soft X-ray tomography (SXT) to image the ultrastructure of whole cryo-preserved cells at a resolution of 25 nm half pitch, is presented.

Tags

Cryo Soft X ray Tomography

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