JoVE Logo
Faculty Resource Center

Sign In

A 3D Cartographic Description of the Cell by Cryo Soft X-ray Tomography

DOI :

10.3791/62190-v

8:47 min

March 15th, 2021

March 15th, 2021

3,079 Views

1MISTRAL beamline, Alba Light Source, Cerdanyola del Valles, Spain, 2Beamline B24, Diamond Light Source, Harwell Science and Innovation Campus, Didcot, UK

Here, a protocol describing the sample preparation and data collection steps required in cryo soft X-ray tomography (SXT) to image the ultrastructure of whole cryo-preserved cells at a resolution of 25 nm half pitch, is presented.

Tags

Cryo Soft X ray Tomography

-- Views

Related Videos

article

The 3D Culturing of Organoids from Murine Intestinal Crypts and a Single Stem Cell for Organoid Research

article

Fluorescence-Based Quantification of Mitochondrial Membrane Potential and Superoxide Levels Using Live Imaging in HeLa Cells

article

Semi-Automated Isolation of the Stromal Vascular Fraction from Murine White Adipose Tissue Using a Tissue Dissociator

article

Direct Infusion Device for Molecule Delivery in Plants

article

Author Spotlight: Exploring Cellular Zinc Regulation Through ZnT1 Functionality

article

Author Spotlight: Advancing Mitochondrial Research – mtHyper7 Biosensor for Subcellular Analysis

article

Imaging ATG9A, a Multi-Spanning Membrane Protein

article

Alignment of Synchronized Time-Series Data Using the Characterizing Loss of Cell Cycle Synchrony Model for Cross-Experiment Comparisons

article

Generation and Manipulation of Rat Intestinal Organoids

article

Author Spotlight: Decoding Corneal Neovascularization with Alkali Burn Model for Future Therapeutic Strategies

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved