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Introducing Point Mutations into Human Pluripotent Stem Cells Using Seamless Genome Editing

DOI :

10.3791/61152-v

9:03 min

May 10th, 2020

May 10th, 2020

3,435 Views

1MRC Centre for Regenerative Medicine, University of Edinburgh

Here, we describe a detailed method for seamless gene editing in human pluripotent stem cells using a piggyBac-based donor plasmid and the Cas9 nickase mutant. Two point mutations were introduced into exon 8 of the hepatocyte nuclear factor 4 alpha (HNF4α) locus in human embryonic stem cells (hESCs).

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Point Mutations

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