JoVE Logo
Faculty Resource Center

Sign In

High-throughput Quantitative Real-time RT-PCR Assay for Determining Expression Profiles of Types I and III Interferon Subtypes

DOI :

10.3791/52650-v

10:00 min

March 24th, 2015

March 24th, 2015

13,019 Views

1Center for Biologics Evaluation and Research, US Food and Drug Administration, 2Center for Drug Evaluation and Research, US Food and Drug Administration

This protocol describes a high-throughput qRT-PCR assay for the analysis of type I and III IFN expression signatures. The assay discriminates single base pair differences between the highly similar transcripts of these genes. Through batch assembly and robotic pipetting, the assays are consistent and reproducible.

Tags

High throughput

-- Views

Related Videos

article

Quantitative High-throughput Single-cell Cytotoxicity Assay For T Cells

article

Multiplex PCR Assay for Typing of Staphylococcal Cassette Chromosome Mec Types I to V in Methicillin-resistant Staphylococcus aureus

article

Pairwise Growth Competition Assay for Determining the Replication Fitness of Human Immunodeficiency Viruses

article

High Throughput, Real-time, Dual-readout Testing of Intracellular Antimicrobial Activity and Eukaryotic Cell Cytotoxicity

article

A Fluorescence-based Lymphocyte Assay Suitable for High-throughput Screening of Small Molecules

article

Bead Based Multiplex Assay for Analysis of Tear Cytokine Profiles

article

Real-time Quaking-induced Conversion Assay for Detection of CWD Prions in Fecal Material

article

Label-Free Identification of Lymphocyte Subtypes Using Three-Dimensional Quantitative Phase Imaging and Machine Learning

article

A High-throughput Shigella-specific Bactericidal Assay

article

Pan-lyssavirus Real Time RT-PCR for Rabies Diagnosis

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved