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A High Yield and Cost-efficient Expression System of Human Granzymes in Mammalian Cells

9.6K Views

09:16 min

June 10th, 2015

DOI :

10.3791/52911-v

June 10th, 2015


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Granzymes

Chapters in this video

0:05

Title

1:40

Expression of Gzms in HEK293T Cells

3:52

Purification of Gzms from Culture Supernatant by Nickel-IMAC

5:25

Enterokinase (EK) Treatment and Final Clean-up by Cation Exchange Chromatography

7:46

Results: Analysis of Recombinant Granzymes by SDS-PAGE and Colorimetric Assays

8:47

Conclusion

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