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Determination of Fatty Acid Oxidation and Lipogenesis in Mouse Primary Hepatocytes

DOI :

10.3791/52982-v

August 27th, 2015

August 27th, 2015

19,862 Views

1Division of Cardiovascular Medicine, Department of Medicine, University of Massachusetts Medical School

De novo lipogenesis and β-fatty acid oxidation constitute key metabolic pathways in hepatocyte, pathways that are perturbed in several metabolic disorders, including fatty liver disease. Here we demonstrate isolation of mouse primary hepatocytes and describe quantification of β-fatty acid oxidation and lipogenesis.

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Fatty Acid Oxidation

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