Inducing and Characterizing Vesicular Steatosis in Differentiated HepaRG Cells

8.7K Views

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09:15 min

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July 18th, 2019

DOI :

10.3791/59843-v

July 18th, 2019

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Silvia Di Cocco¹^,², Laura Belloni³, Abigail D.G. Nunn³, Debora Salerno³, Silvia Piconese²^,⁴, Massimo Levrero²^,³^,⁵^,⁶, Natalia Pediconi¹^,³

¹Department of Molecular Medicine, Sapienza University, ²Department of Internal Medicine - DMISM, Sapienza University, ³Center for Life Nano Science@Sapienza, Istituto Italiano di Tecnologia, ⁴Pasteur Institute Italy-Fondazione Cenci Bolognetti, ⁵INSERM U1052-Cancer Research Center of Lyon, ⁶Department of Hepatology, Croix Rousse Hospital, Hospices Civils de Lyon

Summary

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Differentiation Medium

Chapters in this video

0:04

Title

0:32

Thawing, Amplification, and Cryopreservation of HepaRG Cells

2:20

Differentiation of HepaRG cells (Day 0 21) and Induction of Vesicular Steatosis (Day 21 26)

4:25

Evaluation of Lipid Overloading and Steatosis Induction: Oil Red O Staining

6:04

Evaluation of Lipid Overloading and Steatosis Induction: Bodipy Staining

7:02

Results: Induction and Quantification of Vesicular Steatosis in HepaRG Cells

8:34

Conclusion

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