JoVE Logo
Faculty Resource Center

Sign In

Rapid In Situ Hybridization using Oligonucleotide Probes on Paraformaldehyde-prefixed Brain of Rats with Serotonin Syndrome

DOI :

10.3791/53165-v

September 23rd, 2015

September 23rd, 2015

8,658 Views

1Ross University School of Veterinary Medicine, 2Charles E. Schmidt College of Medicine, Florida Atlantic University

This protocol describes a rapid and simplified in situ hybridization method ideal forparaformaldehyde-prefixed brain, thus reducing the need for prolonged complex steps while using fresh frozen tissues. The method is validated using the identification of the serotonin 5-HT2A receptor gene htr2a in rats.

Tags

Rapid In Situ Hybridization

-- Views

Related Videos

article

Double Fluorescence in situ Hybridization in Fresh Brain Sections

article

TMS: Using the Theta-Burst Protocol to Explore Mechanism of Plasticity in Individuals with Fragile X Syndrome and Autism

article

Combining Lipophilic dye, in situ Hybridization, Immunohistochemistry, and Histology

article

Complete Spinal Cord Injury and Brain Dissection Protocol for Subsequent Wholemount In Situ Hybridization in Larval Sea Lamprey

article

Detection of Axonally Localized mRNAs in Brain Sections Using High-Resolution In Situ Hybridization

article

Combining Double Fluorescence In Situ Hybridization with Immunolabelling for Detection of the Expression of Three Genes in Mouse Brain Sections

article

Recording EEG in Freely Moving Neonatal Rats Using a Novel Method

article

Two-photon Imaging of Microglial Processes' Attraction Toward ATP or Serotonin in Acute Brain Slices

article

Advanced Diffusion Imaging in The Hippocampus of Rats with Mild Traumatic Brain Injury

article

Induction of Diffuse Axonal Brain Injury in Rats Based on Rotational Acceleration

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved