This work was supported by a Programmi di Ricerca Scientifica di Rilevante Interesse Nazionale (PRIN) grant from Ministero Istruzione Universit&#224; e Ricerca (PRIN 2010, protocol number: 2010XPMFW4_009) awarded to GdP. We are also grateful to Caterina Bertini and Raffaella Marino for proofreading the manuscript and performing in the video.

Ethics statement: All procedures described in this protocol were developed and tested following ethical approval from the Ethics Committee of the Psychology Department of Bologna University (see also the Declaration of Helsinki23,53).
1. Participants
<ol>
	<li>Select a sample of healthy young adult females.
	<ol>
		<li>Recruit participants who are not on a diet, not taking psychoactive drugs, free of current or past psychiatric or neurological illness as determined by history, and na&#239;ve as to the purpose of the experiment.</li>
	</ol>
	</li>
	<li>Invite volunteers to sit in a quiet room and collect their demographics, including gender, age, level of education, fasting hours, and height and weight.</li>
	<li>Calculate participants&#39; body mass index (BMI)45,54,55.</li>
</ol>
2. Hunger and Motivation Ratings
<ol>
	<li>Collect subjects&#39; self-ratings about their feeling of hunger at that very moment on an 11-point Likert scale56,57.</li>
	<li>Use a similar scale as the one used in the previous step for self-rating the motivation to consume at that very moment six foods, one by one. Choose these foods among those with high appetitive value58-62 in the female population (based on the cultural context).
	<ol>
		<li>Display the six foods in pictures, randomly, by using a slide show presentation program, so that their appearance and size match as much as possible those in the real word.</li>
	</ol>
	</li>
</ol>
3. Food Stimuli Selection
<ol>
	<li>Select, individually for each participant, the two highest rated food items in the previous step.
	<ol>
		<li>Make sure, at the group level, that participants are equally motivated to eat the two foods, namely, that there is no significant difference between the two items in the motivation rating (see steps 2.2 and 2.2.1).</li>
	</ol>
	</li>
</ol>
4. Errors as a Way to Enhance Cognitive Control
<ol>
	<li>Use the Error task24 modified as follows to associate the two preselected stimuli with two different error rates.
	<ol>
		<li>Program and run the task with an appropriate stimulus delivery software, such as E-Prime. The task should last at least 15 min. See Supplemental Code Files for an example script.</li>
	</ol>
	</li>
	<li>Include two conditions in which one predicts (i.e., it cues) a Low probability of committing an Error (LE), namely, in which participants will make a small number of errors, and one predicts a High probability of committing an Error (HE), namely, in which participants will make a larger number of errors (see steps 4.5.2 and 4.5.3).
	<ol>
		<li>Use one of the two preselected food items as the cue for the LE condition (LEFood); use the other food item as the cue for the HE condition (HEFood). Intersperse, randomly, the same number of trials for both conditions.</li>
	</ol>
	</li>
	<li>Set the task so that each trial starts with a grayscale picture of one of the two food items, that, after 1,000 msec, becomes colored (Go signal), thus requiring the subject to respond.
	<ol>
		<li>For 33% of the total number of trials, superimpose a red circle on the colored image (Stop signal) after a variable stop-signal delay (SSD) (see section 4.4) relative to the Go signal onset, thus requiring the subject not to respond.</li>
		<li>Once the Stop signal appears, leave both Go and Stop signals on the screen for a maximum response time of 1,000 msec after the Go signal onset (time window in which subjects are allowed to respond).</li>
		<li>After giving a feedback (see step 4.4.), separate the trials with a blank screen of variable interstimulus interval (500-2,000 msec).</li>
	</ol>
	</li>
	<li>Instruct participants to respond to the Go signal as quickly as possible via button press, and to refrain when a Stop signal occurs. Instruct them about all possible feedback (i.e., &#39;&#39;Correct!&#39;&#39;, &#39;&#39;Error!&#39;&#39;, &#39;&#39;Too early!&#39;&#39;).</li>
	<li>Define the error likelihoods for the LE and the HE conditions by changing their SSDs independently, by the means of a staircase algorithm, based on participant&#39;s performance. The LE has shorter SSDs, whereas the HE has longer SSDs24.
	<ol>
		<li>Start the SSD at 200 msec in both LE and HE after the first Go signal onset.</li>
		<li>During the LE, increase the SSD by 5 msec on the subsequent trial if the subject succeeds in avoiding the button press after the Stop signal; decrease the SSD by 50 msec on the subsequent trial if the subject fails.</li>
		<li>During the HE, increase the SSD by 50 msec on the next trial if the subject succeeds, otherwise, decrease the SSD by 50 msec on the next trial if the subject fails63.</li>
	</ol>
	</li>
	<li>For the analyses, compare Go and Stop trials and LE and HE conditions for accuracy and response time24. Do not consider missed responses. Use Statistica or any other statistical software to analyze the data.</li>
</ol>
5. Intertemporal Choice as a Measure of Cognitive Control
<ol>
	<li>Administer participants two Temporal Discounting tasks, separately and in a counterbalanced order, soon after the Error task ends (see below for details). Measure in one Temporal Discounting task the subjective value for the LEFood, and in the other the subjective value for the HEFood. Run the tasks on E-Prime or any other stimulus delivery software. See Supplemental Code Files for an example script.
	<ol>
		<li>In each task, present participants, on each trial, a choice between 40 units of fictive food25,64,65 that can be obtained after a variable delay (i.e., 2 days, 2 weeks, 1 month, 3 months, 6 months, and 1 year, for a total of six delay conditions25,66,67) and a smaller amount of the same food available in the immediate present.</li>
		<li>Include five choices in each delay condition. Deliver blocks of choices pertaining to the six delay conditions in a random order across subjects.</li>
	</ol>
	</li>
	<li>Instruct participants to make a choice between the two options by imagining to receive the specified amount of reward at the specified time, and to indicate their preference by pressing one of two buttons25,68.
	<ol>
		<li>Make clear to participants that they are making choices toward hypothetical foods; there are no correct or incorrect choices but they are free to choose the option they prefer the most; they have no time limit to decide, but they should make their choice as soon as they feel to prefer one reward more than the other.</li>
	</ol>
	</li>
	<li>Program the tasks so that, for each delay condition, the amount of the smaller-immediate option is adjusted, trial by trial, based on participants&#39; previous choice, using a titration procedure that converges on the amount of the immediate reward that has equivalent subjective value as the delayed reward25,67 (see steps 5.3.1 to 5.3.5).
	<ol>
		<li>First, always submit participants, for each delay condition, to a choice between 20 units (bites) of food available &#34;now&#34; and 40 units of the same food available at that specific delay67, so that, in the first trial of each delay condition participants always face a choice between these two specific amounts67.</li>
		<li>Then, decrease, after the first choice, the amount of the smaller option on the next trial if participant chose the smaller option in the previous trial, otherwise increase the amount of the smaller option on the next trial if the participant chose the larger option in the previous trial.</li>
		<li>Reduce the size of the adjustment of the smaller option with successive choices: The first adjustment is half of the difference between the smaller and the larger rewards, whereas for subsequent choices it is half of the previous adjustment23.</li>
		<li>Repeat the procedure in the above step until the participant has made five choices at one specific delay condition, after which the participant begins a new series of choices at another delay condition.</li>
		<li>For each trial in a block, consider the smaller immediate amount as the best guess as to the subjective value of the larger later reward. Therefore, take the immediate amount that would have been presented on the sixth trial of a delay block as the estimate of the subjective value of the later reward at that delay and use it for the analysis (i.e., the so-called indifference point; see step 5.6)25.</li>
	</ol>
	</li>
	<li>Fit, through nonlinear, least-squares analysis, the hyperbolic equation V = 1/(1 + kT) (i.e., V = subjective value; k = constant of the best fitting; T = time delay in days) to participants&#39; indifference points23, independently for each Temporal Discounting task. Do this in order to calculate the discount rate (k) of the subjective value for each reward under consideration as a function of time69-71. Use Statistica or any other statistical software to perform this non-linear estimation.</li>
	<li>Log-transform k values in order to normalize data, and then run parametric analyses23,25. 
	Note: A larger k value corresponds to a steeper discounting function, thereby indicating that the subjects are more inclined to choose the smaller-immediate option over the larger later one.</li>
	<li>Compare the k values for the LEFood and the HEFood by correcting for hunger rating, self-report questionnaire score of sensitivity to external edible cues (see step 6), and BMI45,54,55. Use Statistica or any other statistical software to analyze the data.</li>
</ol>
6. External Eating Behavior Subscale of the Dutch Eating Behavior Questionnaire (DEBQEEB)
<ol>
	<li>Use the DEBQEEB72 to measure volunteers&#39; sensitivity to external edible cues, to control for possible confounding during the experiment.</li>
</ol>
7. Debriefing
<ol>
	<li>Debrief participants as to the purpose of the experiment at the end of the session. Ask them if they were somehow aware of the error manipulation during the Error task and if they think they chose somehow in a different way during the Temporal Discounting tasks according to the two different foods. 
	Note: Any impressions or feelings about the entire procedure (e.g., whether they think there was an association between the two main tasks) are welcome.</li>
</ol>

The authors have nothing to disclose.

This article describes in detail a novel protocol aimed at reducing impulsive food choice in healthy young adult women. Critical steps in this protocol include sampling participants from the healthy female population, collecting self-report hunger level at the time of the experiment, selecting two foods with equivalent incentive value for each subject, submitting participants to an error task where each of two different error likelihoods (high and low, randomly interspersed across trials) are associated with one of the t...

Nowadays, it is crucial to help people face the rise of eating disorders1-4. These disorders reflect an overestimation of the incentive motivation associated with appetitive food, which induces individuals to seek and consume it as soon as possible (this has been shown especially with sweet high-fat foods5-6). This happens at the expense of future benefits that can result from being on a diet for a while, but for which the ability to exert eating control is necessary7-8. Indeed, people showing these abnormal behaviors have increased attentional bias toward edible cues9-10 and experience enhanced incentive value for primary rewards11. Moreover, even just looking at appetizing foodstuffs can cue the desire for consuming the food immediately, both in individuals with eating disorders and in the normal population12-13. In order to refrain from immediate gratification and not forego the long-term outcome (e.g., losing weight after a few months of diet), one must exercise great self-controlled and resist the innate, evolutionarily determined impulse to give into temptation and consume immediately. Exerting self-control, a concept that is interrelated to the notion of cognitive control in the field of the Neuroscience, means that one is capable of overcoming innate impulses for further consideration and, possibly, implementation of other, more appropriate behaviors14.
How do individuals engage in self-control strategies? Research has highlighted over the years that the capacity to refrain from automatic responses is heightened in error-full contexts15. Errors are well considered highly arousing and aversive events that, when encountered, elicit compensatory responses16. Specifically, they cue failure/loss in both performance and utility, thereby signaling that one needs to adjust levels of control over current and future behavior accordingly17. Moreover, errors can cue aversive learning, as a warning way to escape from error-prone, maladaptive behaviors, thus inducing the implementation of optimal choice responses18-21.
The present protocol shows how the association between tasty food items and errors can signal that engaging in a certain behavior would lead to a cost (i.e., reward loss), thus encouraging the implementation of compensatory self-control strategies, and thereby reducing impulsive food choice20,22. In the present protocol adapted from23, participants are preliminarily asked to self-report their hunger level at the time of the experiment and to rate six different food items. Based on the ratings, two foods with equivalent incentive value for each subject are selected for the subsequent task. Then, participants perform an error task (see reference24) in which the two previously selected food items cue different error rates (high and low) associated with performance: The error task is programmed so that in one trial condition, cued by one food, participants make a small number of errors, and in the other trial condition, cued by the other food, they make a much larger number of errors. Afterwards, participants&#39; intertemporal choice for each of the two primary rewards is measured (adapted from reference25). The ability to seek larger but delayed reinforcements instead of sooner gratification, which is crucial when facing tempting foods while being on a diet, is indeed crisply captured by intertemporal choice paradigms26. The longer the time one needs to wait for a good to be received and consumed, the more the subjective evaluation of this potential reward is weakened (i.e., the so-called temporal discounting phenomenon27-34). Poor decisions (i.e., higher propensity to choose close gratifications, namely increased temporal discounting for future gains) are considered as a core feature of impulsivity35 and a landmark of numerous disorders, including drug addiction and obesity36-45. After undergoing the procedure described in this protocol, participants show reduced impatient choice for the stimulus cueing high error rate, selectively. The effect is more evident when the hunger level reported by the subjects is low23. This occurs because hunger affects the immediate evaluation of food items46-49 by heightening the motivational value of primary rewards and, in turn, the discount rate of future amounts of those rewards7,50-52.
The advantage of this method first lies in its easy applicability. The error training preceding the intertemporal decision tasks is almost completely effortless, making it possible to be employed in diverse clinical settings. Second, the method produces the desired effect with hypothetical edible items, without the need to use real food. Third, participants were mainly unaware of the food-error association, making the subsequent effect on food preferences genuine, which might affect food decisions reliably in real life as well. Finally, participants tested in the study23 were all young females, but there is good reason to speculate that the effect of food-error pairing on intertemporal decisions would be the same on young males too, mainly because subjects in the present study were unaware of the intended effect.

<table border="0" cellpadding="0" cellspacing="0" width="573">
	<tbody>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">E-Prime</td>
			<td style="width:71px;">PST</td>
			<td style="width:119px;"></td>
			<td style="width:167px;">Stimulus Delivery Software</td>
		</tr>
		<tr height="21">
			<td height="21" style="height:21px;width:216px;">Statistica</td>
			<td style="width:71px;">Statsoft</td>
			<td style="width:119px;"></td>
			<td>Statistical Software</td>
		</tr>
	</tbody>
</table>

errors as a means of reducing impulsive food choice

Nowadays, the increasing incidence of eating disorders due to poor self-control has given rise to increased obesity and other chronic weight problems, and ultimately, to reduced life expectancy. The capacity to refrain from automatic responses is usually high in situations in which making errors is highly likely. The protocol described here aims at reducing imprudent preference in women during hypothetical intertemporal choices about appetitive food by associating it with errors. First, participants undergo an error task where two different edible stimuli are associated with two different error likelihoods (high and low). Second, they make intertemporal choices about the two edible stimuli, separately. As a result, this method decreases the discount rate for future amounts of the edible reward that cued higher error likelihood, selectively. This effect is under the influence of the self-reported hunger level. The present protocol demonstrates that errors, well known as motivationally salient events, can induce the recruitment of cognitive control, thus being ultimately useful in reducing impatient choices for edible commodities.

Representative results from the application of the protocol described above are reported here.
Error task
The validity of the Error task has been determined by the following results. Concerning the percentage of errors committed by participants, they showed a significantly higher number of errors in the HE than in the LE condition, a significantly higher number of erro...

Watch this Scientific Journal Video about Errors as a Means of Reducing Impulsive Food Choice at JoVE.com

Errors as a Means of Reducing Impulsive Food Choice

Giving in to temptation of tasty food may result in long-term overweight problems. This protocol describes how to reduce imprudent preference for edible commodities during hypothetical intertemporal choices in women by associating them with errors.

Nowadays, the increasing incidence of eating disorders due to poor self-control has given rise to increased obesity and other chronic weight problems, and ...

errors-as-a-means-of-reducing-impulsive-food-choice

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8.5K Views.  Justus-Liebig-Universität Giessen. Giving in to temptation of tasty food may result in long-term overweight problems. This protocol describes how to reduce imprudent preference for edible commodities during hypothetical intertemporal choices in women by associating them with errors.

Video: Errors as a Means of Reducing Impulsive Food Choice

The Crossmodal Congruency Task as a Means to Obtain an Objective Behavioral Measure in the Rubber Hand Illusion Paradigm

The rubber hand illusion (RHI) is a popular experimental paradigm. Participants view touch on an artificial rubber hand while the participants' own hidden hand is touched. If the viewed and felt touches are given at the same time then this is sufficient to induce the compelling experience that the rubber hand is one's own hand. The RHI can be used to investigate exactly how the brain constructs distinct body representations for one's own body. Such representations are crucial for successful interactions with the external world. To obtain a subjective measure of the RHI, researchers typically ask participants to rate statements such as "I felt as if the rubber hand were my hand". Here we demonstrate how the crossmodal congruency task can be used to obtain an objective behavioral measure within this paradigm.
The variant of the crossmodal congruency task we employ involves the presentation of tactile targets and visual distractors. Targets and distractors are spatially congruent (i.e. same finger) on some trials and incongruent (i.e. different finger) on others. The difference in performance between incongruent and congruent trials - the crossmodal congruency effect (CCE) - indexes multisensory interactions. Importantly, the CCE is modulated both by viewing a hand as well as the synchrony of viewed and felt touch which are both crucial factors for the RHI.
The use of the crossmodal congruency task within the RHI paradigm has several advantages. It is a simple behavioral measure which can be repeated many times and which can be obtained during the illusion while participants view the artificial hand. Furthermore, this measure is not susceptible to observer and experimenter biases. The combination of the RHI paradigm with the crossmodal congruency task allows in particular for the investigation of multisensory processes which are critical for modulations of body representations as in the RHI.

We demonstrate how an objective measure can be employed in the widely employed rubber hand illusion paradigm. This measure is obtained by modifying the well-established crossmodal congruency task. This task allows the investigation of multisensory processes which are critical for modulations of body representations as in the rubber hand illusion.

The rubber hand illusion (RHI) is a popular experimental paradigm. Participants view touch on an artificial rubber hand while the participants' own hidden ...

Studying Food Reward and Motivation in Humans

A key challenge in studying reward processing in humans is to go beyond subjective self-report measures and quantify different aspects of reward such as hedonics, motivation, and goal value in more objective ways. This is particularly relevant for the understanding of overeating and obesity as well as their potential treatments. In this paper are described a set of measures of food-related motivation using handgrip force as a motivational measure. These methods can be used to examine changes in food related motivation with metabolic (satiety) and pharmacological manipulations and can be used to evaluate interventions targeted at overeating and obesity. However to understand food-related decision making in the complex food environment it is essential to be able to ascertain the reward goal values that guide the decisions and behavioral choices that people make. These values are hidden but it is possible to ascertain them more objectively using metrics such as the willingness to pay and a method for this is described. Both these sets of methods provide quantitative measures of motivation and goal value that can be compared within and between individuals.

This article describes a set of methods for the measurement of food related motivation and food related goal values in humans.

A key challenge in studying reward processing in humans is to go beyond subjective self-report measures and quantify different aspects of reward such as ...

The 5-Choice Serial Reaction Time Task: A Task of Attention and Impulse Control for Rodents

This protocol describes the 5-choice serial reaction time task, which is an operant based task used to study attention and impulse control in rodents. Test day challenges, modifications to the standard task, can be used to systematically tax the neural systems controlling either attention or impulse control. Importantly, these challenges have consistent effects on behavior across laboratories in intact animals and can reveal either enhancements or deficits in cognitive function that are not apparent when rats are only tested on the standard task. The variety of behavioral measures that are collected can be used to determine if other factors (i.e., sedation, motivation deficits, locomotor impairments) are contributing to changes in performance. The versatility of the 5CSRTT is further enhanced because it is amenable to combination with pharmacological, molecular, and genetic techniques.

This protocol describes the 5-choice serial reaction time task, which is an operant based task used to study attention and impulse control in rodents. Test day challenges, which are modifications of the standard task, increase flexibility of the task and can be combined with other manipulations to more fully characterize behavior.

This protocol describes the 5-choice serial reaction time task, which is an operant based task used to study attention and impulse control in rodents. ...

The Use of the Puzzle Box as a Means of Assessing the Efficacy of Environmental Enrichment

Environmental enrichment can dramatically influence the development and function of neural circuits. Further, enrichment has been shown to successfully delay the onset of symptoms in models of Huntington&#8217;s disease 1-4, suggesting environmental factors can evoke a neuroprotective effect against the progressive, cellular level damage observed in neurodegenerative disorders. The ways in which an animal can be environmentally enriched, however, can vary considerably. Further, there is no straightforward manner in which the effects of environmental enrichment can be assessed: most methods require either fairly complicated behavioral paradigms and/or postmortem anatomical/physiological analyses. This protocol describes the use of a simple and inexpensive behavioral assay, the Puzzle Box 5-7 as a robust means of determining the efficacy of increased social, sensory and motor stimulation on mice compared to cohorts raised in standard laboratory conditions. This simple problem solving task takes advantage of a rodent&#8217;s innate desire to avoid open enclosures by seeking shelter. Cognitive ability is assessed by adding increasingly complex impediments to the shelter&#8217;s entrance. The time a given subject takes to successfully remove the obstructions and enter the shelter serves as the primary metric for task performance. This method could provide a reliable means of rapidly assessing the efficacy of different enrichment protocols on cognitive function, thus paving the way for systematically determining the role specific environmental factors play in delaying the onset of neurodevelopmental and neurodegenerative disease.

Environmental enrichment provides a potential protective effect against neurodegenerative disorders. Currently, however, there is no easy way of determining the efficacy of enrichment procedures. This protocol describes a simple &#8220;Puzzle Box&#8221; method for assessing an animal&#8217;s cognitive function, in order to reveal the effectiveness of environmental enrichment.

Environmental enrichment can dramatically influence the development and function of neural circuits.  Further, enrichment has been shown to successfully ...

A Treatment Package without Escape Extinction to Address Food Selectivity

Feeding difficulties and feeding disorders are a commonly occurring problem for young children, particularly children with developmental delays including autism. Behavior analytic interventions for the treatment of feeding difficulties oftentimes include escape extinction as a primary component of treatment. The use of escape extinction, while effective, may be problematic as it is also associated with the emergence of challenging behavior (e.g., extinction burst). Such challenging behavior may be an acceptable side effect in treatment cases where feeding problems are severe and chronic (e.g., failure to thrive). However, in more acute cases (e.g., selective eating), the negative side effect may be unwarranted and undesired. More recent research on the behavioral treatment of food selectivity has begun to evaluate treatments for feeding difficulties that do not include escape extinction (e.g., demand fading, behavioral momentum), with some success. However, research to date reveals individual differences in responsiveness to such treatments and no clear preferable treatment has emerged. This manuscript describes a multi-component treatment package that includes shaping, sequential presentation and simultaneous presentation, for the treatment of food selectivity in four young children with developmental delays. This treatment package extends the literature on the behavioral treatment for food selectivity and offers a multi-component treatment protocol that may be clinically applicable across a range of treatment scenarios and settings.

Feeding difficulties are a common problem for children with developmental disorders, including autism, and behavioral interventions often include escape extinction. Recent research has begun to evaluate treatments that do not include escape extinction. This manuscript describes a multicomponent treatment package that does not use escape extinction to treat feeding difficulties.

Feeding difficulties and feeding disorders are a commonly occurring problem for young children, particularly children with developmental delays including ...

Determining Ultrasonic Vocalization Preferences in Mice using a Two-choice Playback Test

Mice emit ultrasonic vocalizations (USVs) during a variety of conditions, such as pup isolation and adult social interactions. These USVs differ with age, sex, condition, and genetic background of the emitting animal. Although many studies have characterized these differences, whether receiver mice can discriminate among objectively different USVs and show preferences for particular sound traits remains to be elucidated. To determine whether mice can discriminate between different characteristics of USVs, a playback experiment was developed recently, in which preference responses of mice to two different USVs could be evaluated in the form of a place preference.
First, USVs from mice were recorded. Then, the recorded USVs were edited, trimmed accordingly, and exported as stereophonic sound files. Next, the USV amplitudes generated by the two ultrasound emitters used in the experiment were adjusted to the same sound pressure level. Nanocrystalline silicon thermo-acoustic emitters were used to play the USVs back. Finally, to investigate the preference of subject mice to selected USVs, pairs of two differing USV signals were played back simultaneously in a two-choice test box. By repeatedly entering a defined zone near an ultrasound emitter and searching the wire mesh in front of the emitter, the mouse reveals its preference for one sound over another. This model allows comparing the attractiveness of the various features of mouse USVs, in various contexts.

Ultrasonic vocalizations (USVs) in mice differ depending on age, sex, condition, and genetic background. Using two ultrasound emitters broadcasting simultaneously in different locations, this two-choice test can evaluate murine recognition and preference responses to different characteristics of USVs.

Mice emit ultrasonic vocalizations (USVs) during a variety of conditions, such as pup isolation and adult social interactions. These USVs differ with age, ...

Protocol for Data Collection and Analysis Applied to Automated Facial Expression Analysis Technology and Temporal Analysis for Sensory Evaluation

We demonstrate a method for capturing emotional response to beverages and liquefied foods in a sensory evaluation laboratory using automated facial expression analysis (AFEA) software. Additionally, we demonstrate a method for extracting relevant emotional data output and plotting the emotional response of a population over a specified time frame. By time pairing each participant's treatment response to a control stimulus (baseline), the overall emotional response over time and across multiple participants can be quantified. AFEA is a prospective analytical tool for assessing unbiased response to food and beverages. At present, most research has mainly focused on beverages. Methodologies and analyses have not yet been standardized for the application of AFEA to beverages and foods; however, a consistent standard methodology is needed. Optimizing video capture procedures and resulting video quality aids in a successful collection of emotional response to foods. Furthermore, the methodology of data analysis is novel for extracting the pertinent data relevant to the emotional response. The combinations of video capture optimization and data analysis will aid in standardizing the protocol for automated facial expression analysis and interpretation of emotional response data.

A protocol for capturing and statistically analyzing emotional response of a population to beverages and liquefied foods in a sensory evaluation laboratory using automated facial expression analysis software is described.

We demonstrate a method for capturing emotional response to beverages and liquefied foods in a sensory evaluation laboratory using automated facial ...

Feeding Experimentation Device (FED): Construction and Validation of an Open-source Device for Measuring Food Intake in Rodents

Food intake measurements are essential for many research studies. Here, we provide a detailed description of a novel solution for measuring food intake in mice: the Feeding Experimentation Device (FED). FED is an open-source system that was designed to facilitate flexibility in food intake studies. Due to its compact and battery powered design, FED can be placed within standard home cages or other experimental equipment. Food intake measurements can also be synchronized with other equipment in real-time via FED's transistor-transistor logic (TTL) digital output, or in post-acquisition processing as FED timestamps every event with a real-time clock. When in use, a food pellet sits within FED's food well where it is monitored via an infrared beam. When the pellet is removed by the mouse, FED logs the timestamp onto its internal secure digital (SD) card and dispenses another pellet. FED can run for up to 5 days before it is necessary to charge the battery and refill the pellet hopper, minimizing human interference in data collection. Assembly of FED requires minimal engineering background, and off-the-shelf materials and electronics were prioritized in its construction. We also provide scripts for analysis of food intake and meal patterns. Finally, FED is open-source and all design and construction files are online, to facilitate modifications and improvements by other researchers.

Feeding Experimentation Device FED: Construction and Validation of an Open-source Device for Measuring Food Intake in Rodents

Feeding Experimentation Device (FED) is an open-source device for measuring food intake in mice. FED can also synchronize food intake measurements with other techniques via a real-time digital output. Here, we provide a step-by-step tutorial for the construction, validation, and usage of FED.

Food intake measurements are essential for many research studies. Here, we provide a detailed description of a novel solution for measuring food intake in ...

Ultrasound Images of the Tongue: A Tutorial for Assessment and Remediation of Speech Sound Errors

Diagnostic ultrasound imaging has been a common tool in medical practice for several decades. It provides a safe and effective method for imaging structures internal to the body. There has been a recent increase in the use of ultrasound technology to visualize the shape and movements of the tongue during speech, both in typical speakers and in clinical populations. Ultrasound imaging of speech has greatly expanded our understanding of how sounds articulated with the tongue (lingual sounds) are produced. Such information can be particularly valuable for speech-language pathologists. Among other advantages, ultrasound images can be used during speech therapy to provide (1) illustrative models of typical (i.e.&#160;&#34;correct&#34;) tongue configurations for speech sounds, and (2) a source of insight into the articulatory nature of deviant productions. The images can also be used as an additional source of feedback for clinical populations learning to distinguish their better productions from their incorrect productions, en route to establishing more effective articulatory habits.
Ultrasound feedback is increasingly used by scientists and clinicians as both the expertise of the users increases and as the expense of the equipment declines. In this tutorial, procedures are presented for collecting ultrasound images of the tongue in a clinical context. We illustrate these procedures in an extended example featuring one common error sound, American English /r/. Images of correct and distorted /r/ are used to demonstrate (1) how to interpret ultrasound images, (2) how to assess tongue shape during production of speech sounds, (3), how to categorize tongue shape errors, and (4), how to provide visual feedback to elicit a more appropriate and functional tongue shape. We present a sample protocol for using real-time ultrasound images of the tongue for visual feedback to remediate speech sound errors. Additionally, example data are shown to illustrate outcomes with the procedure.

Ultrasound imaging can be used to display the shape and movements of the tongue in real time during speech. The images can be used to determine the nature of speech sound errors. Visual feedback of the tongue can be used to facilitate improvements in speech sound production in clinical populations.

Diagnostic ultrasound imaging has been a common tool in medical practice for several decades. It provides a safe and effective method for imaging ...

Reducing State Anxiety Using Working Memory Maintenance

The purpose of this protocol is to explain how to examine the relationship between working memory processes and anxiety by combining the Sternberg Working Memory (WM) and the threat of shock paradigms. In the Sternberg WM paradigm, subjects are required to maintain a series of letters in the WM for a brief interval and respond by identifying whether the position of a given letter in the series matches a numerical prompt. In the threat of shock paradigm, subjects are exposed to alternating blocks where they are either at risk of receiving unpredictable presentations of a mild electric shock or are safe from the shock. Anxiety is probed throughout the safe and threat blocks using the acoustic startle reflex, which is potentiated under threat (Anxiety-Potentiated Startle (APS)). By conducting the Sternberg WM paradigm during the threat of shock and probing the startle response during either the WM maintenance interval or the intertrial interval, it is possible to determine the effect of WM maintenance on APS.

This protocol demonstrates how to measure anxiety-potentiated startle during the Sternberg Working Memory paradigm.

The purpose of this protocol is to explain how to examine the relationship between working memory processes and anxiety by combining the Sternberg Working ...