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Optimization of the Wound Scratch Assay to Detect Changes in Murine Mesenchymal Stromal Cell Migration After Damage by Soluble Cigarette Smoke Extract

DOI :

10.3791/53414-v

December 3rd, 2015

December 3rd, 2015

16,674 Views

1Department of Biology, College of the Holy Cross

The capacity to migrate is a key function of many different cell types, including mesenchymal stromal cells (MSCs). However, quantifying alterations in migratory capacity after damage is challenging. This protocol describes an easily adaptable migration assay that uses rigorous statistics to quantify changes in MSC migratory capacity after damage.

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Optimized Wound Scratch Assay

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