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Isolation and Culture of Dissociated Sensory Neurons From Chick Embryos

DOI :

10.3791/51991-v

September 24th, 2014

September 24th, 2014

13,979 Views

1Department of Natural Sciences, Assumption College

Cell culture models provide detailed control over environmental conditions and thus provide a powerful platform to elucidate numerous aspects of neuronal cell biology. We describe a rapid, inexpensive, and reliable method to isolate, dissociate, and culture sensory neurons from chick embryos. Details of substrata preparation and immunocytochemistry are also provided.

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Isolation

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