JoVE Logo
Faculty Resource Center

Sign In

Generation of Parabiotic Zebrafish Embryos by Surgical Fusion of Developing Blastulae

DOI :

10.3791/54168-v

June 11th, 2016

June 11th, 2016

11,125 Views

1Division of Hematology/Oncology, Boston Children’s Hospital, 2Harvard Medical School, 3Division of Hematology, Department of Medicine, Brigham and Women’s Hospital, 4Harvard Stem Cell Institute, 5Broad Institute of Massachusetts Institute of Technology, 6Howard Hughes Medical Institute, 7Division of Hematologic Malignancies, Dana-Farber Cancer Institute

This protocol provides step-by-step instruction on how to generate parabiotic zebrafish embryos of different genetic backgrounds. When combined with the unparalleled imaging capabilities of the zebrafish embryo, this method provides a uniquely powerful means to investigate cell-autonomous versus non-cell-autonomous functions for candidate genes of interest.

Tags

Parabiotic Zebrafish Embryos

-- Views

Related Videos

article

Mechanical Vessel Injury in Zebrafish Embryos

article

Isolation and Characterization of Single Cells from Zebrafish Embryos

article

Generation of Induced Pluripotent Stem Cells from Human Melanoma Tumor-infiltrating Lymphocytes

article

Affinity Labeling Detection of Endogenous Receptors from Zebrafish Embryos

article

Ploidy Manipulation of Zebrafish Embryos with Heat Shock 2 Treatment

article

Biosensing Motor Neuron Membrane Potential in Live Zebrafish Embryos

article

Induction of Hypoxia in Living Frog and Zebrafish Embryos

article

Development of an In Vitro Assay to Quantitate Hematopoietic Stem and Progenitor Cells (HSPCs) in Developing Zebrafish Embryos

article

Visualization of Cellular Electrical Activity in Zebrafish Early Embryos and Tumors

article

Surgical Size Reduction of Zebrafish for the Study of Embryonic Pattern Scaling

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved