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Induction of Hypoxia in Living Frog and Zebrafish Embryos

DOI :

10.3791/55710-v

June 26th, 2017

June 26th, 2017

9,451 Views

1Department of Physiology, Development and Neuroscience, University of Cambridge

We introduce a novel hypoxic chamber system for use with aquatic organisms such as frog and zebrafish embryos. Our system is simple, robust, cost-effective and allows the induction and sustainment of hypoxia in vivo and for up to 48 h. We present 2 reproducible methods to monitor the effectiveness of hypoxia.

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Keywords Hypoxia

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