JoVE Logo
Faculty Resource Center

Sign In

Biochemical Assays for Analyzing Activities of ATP-dependent Chromatin Remodeling Enzymes

DOI :

10.3791/51721-v

October 25th, 2014

October 25th, 2014

14,574 Views

1Stowers Institute for Medical Research, 2Department of Biochemistry & Molecular Biology, Kansas University Medical Center

Here we describe biochemical assays that can be used to characterize ATP-dependent chromatin remodeling enzymes for their abilities to 1) catalyze ATP-dependent nucleosome sliding, 2) engage with nucleosome substrates, and 3) hydrolyze ATP in a nucleosome- or DNA-dependent manner.

Tags

Biochemical Assays

-- Views

Related Videos

article

A Chromatin Assay for Human Brain Tissue

article

Zebrafish Brain Ventricle Injection

article

Photobleaching Assays (FRAP & FLIP) to Measure Chromatin Protein Dynamics in Living Embryonic Stem Cells

article

Quantitative Comparison of cis-Regulatory Element (CRE) Activities in Transgenic Drosophila melanogaster

article

Blood Collection for Biochemical Analysis in Adult Zebrafish

article

Extraction of Tissue Antigens for Functional Assays

article

Biochemical and High Throughput Microscopic Assessment of Fat Mass in Caenorhabditis Elegans

article

Biochemical Titration of Glycogen In vitro

article

Viability Assays for Cells in Culture

article

Generation and Purification of Human INO80 Chromatin Remodeling Complexes and Subcomplexes

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved