Sinoatrial Node Digestion and Sinoatrial Node Myocyte Dissociation
6:29
Sinoatrial Node Calcium Re-adaptation
7:36
Results: Electrophysiological Characterization of Cultured Sinoatrial Myocytes
8:27
Conclusion
필기록
The overall goal of this procedure is to isolate spontaneously active sinoatrial node myocytes from mice for short or medium-term electrophysiological or imaging experiments. Isolated sinoatrial node myocytes are important for the study of cardiac
이 콘텐츠에 액세스하려면 로그인하거나 무료 평가판을 시작하세요
Methods are demonstrated for the isolation of sinoatrial node myocytes (SAMs) from adult mice for patch clamp electrophysiology or imaging studies. Isolated cells can be used directly or can be maintained in culture to permit expression of proteins of interest, such as genetically encoded reporters.