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Preparation and In Vivo Use of an Activity-based Probe for N-acylethanolamine Acid Amidase

DOI :

10.3791/54652-v

November 23rd, 2016

November 23rd, 2016

9,455 Views

1Drug Discovery and Development, Istituto Italiano di Tecnologia, 2Departments of Anatomy and Neurobiology, Pharmacology, and Biological Chemistry, University of California, Irvine School of Medicine

Here, we describe the preparation and use of an activity-based probe (ARN14686, undec-10-ynyl-N-[(3S)-2-oxoazetidin-3-yl]carbamate) that allows for the detection and quantification of the active form of the proinflammatory enzyme N-acylethanolamine acid amidase (NAAA), both in vitro and ex vivo.

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Activity based Probe

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