JoVE Logo
Faculty Resource Center

Sign In

Multi-enzyme Screening Using a High-throughput Genetic Enzyme Screening System

DOI :

10.3791/54059-v

August 8th, 2016

August 8th, 2016

8,407 Views

1Synthetic Biology & Bioengineering Research Center, Korea Research Institute of Bioscience and Biotechnology, 2Biosystems and Bioengineering Program, University of Science and Technology, Daejeon, South Korea

This work presents a method of high-throughput screening using a universal genetic enzyme screening system that can be theoretically applied to over 200 enzymes. Here, the single screening system identifies three different enzymes (lipase, cellulase, and alkaline phosphatase) by simply changing the substrate used (p-nitrophenyl acetate, p-nitrophenyl-β-D-cellobioside, and phenyl phosphate).

-- Views

Related Videos

article

An Antimicrobial Fabric Using Nano-Herbal Encapsulation of Essential Oils

article

Semi-Automated Isolation of the Stromal Vascular Fraction from Murine White Adipose Tissue Using a Tissue Dissociator

article

Effective Rapid Blood Perfusion in Xenopus

article

Direct Infusion Device for Molecule Delivery in Plants

article

Two-Step Tag-Free Isolation of Mitochondria for Improved Protein Discovery and Quantification

article

Author Spotlight: Exploring Cellular Zinc Regulation Through ZnT1 Functionality

article

Alignment of Synchronized Time-Series Data Using the Characterizing Loss of Cell Cycle Synchrony Model for Cross-Experiment Comparisons

article

Author Spotlight: The Production of Recombinant Proteins

article

Author Spotlight: A Selective Luciferase-Based Assay for Monitoring ATG4B 27 Activity in Cells

article

Author Spotlight: Addressing Regulatory Gaps in Molecular Studies by Quantifying Viral Vectors in Complex Matrices

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved