Results: Fluorometric Enzyme Assay for Apolipoprotein N-Acyltransferase
7:09
Conclusion
Transcript
This protocol addresses two important aspects, characterization of an essential integral membrane, apolipoprotein acyltransferase of bacteria, and development of the assay in HDS format to screen for inhibitors of the enzyme. The method is very se
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Presented here is a sensitive fluorescence assay to monitor apolipoprotein N-acyltransferase activity using diacylglyceryl peptide and alkyne-phospholipids as substrates with click-chemistry.