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Isolation and Culture of Primary Endothelial Cells from Canine Arteries and Veins

DOI :

10.3791/54786-v

November 18th, 2016

November 18th, 2016

10,946 Views

1Department of Clinical Sciences of Companion Animals, Faculty of Veterinary Medicine, Utrecht University

Novel isolation methods of primary endothelial cells from blood vessels are needed. This protocol describes a new technique that completely inverts blood vessels of interest, exposing only the endothelial side to enzymatic digestion. The resulting pure endothelial cell culture can be used to study cardiovascular diseases, disease modelling, and angiogenesis.

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Isolation

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