Isolation and Culture of Primary Canine Endothelial Cells
4:30
Characterization of Canine Aortic Endothelial Cells
6:14
Results: Canine Primary Endothelial Cell Isolation and Culture
7:35
Conclusion
필기록
The overall goal of this method is to isolate and culture primary endothelial cells from dogs to study cardiovascular disease in vitro. This method can help answer key questions in the field of cardiovascular research such as the behavior of endot
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Novel isolation methods of primary endothelial cells from blood vessels are needed. This protocol describes a new technique that completely inverts blood vessels of interest, exposing only the endothelial side to enzymatic digestion. The resulting pure endothelial cell culture can be used to study cardiovascular diseases, disease modelling, and angiogenesis.