October 7th, 2020
•This protocol describes transillumination-assisted microdissection of segments of adult mouse seminiferous tubules representing specific stages of seminiferous epithelial cycle, and cell types therein, and subsequent immunostaining of squash preparations and intact tubule segments.
Tags
Related Videos
Step-specific Sorting of Mouse Spermatids by Flow Cytometry
Isolation of Mouse Endometrial Epithelial and Stromal Cells for In Vitro Decidualization
Dissection of Larval Zebrafish Gonadal Tissue
Dissection and Culture of Mouse Embryonic Kidney
Dissection and Coronal Slice Preparation of Developing Mouse Pituitary Gland
A Seminiferous Tubule Squash Technique for the Cytological Analysis of Spermatogenesis Using the Mouse Model
Single-cell Transcriptomic Analyses of Mouse Pancreatic Endocrine Cells
Tissue Preparation and Immunostaining of Mouse Craniofacial Tissues and Undecalcified Bone
Isolation and Staining of Mouse Skin Keratinocytes for Cell Cycle Specific Analysis of Cellular Protein Expression by Mass Cytometry
Dissection, Culture and Analysis of Primary Cranial Neural Crest Cells from Mouse for the Study of Neural Crest Cell Delamination and Migration
ABOUT JoVE
Copyright © 2024 MyJoVE Corporation. All rights reserved