Detection of Inter-chromosomal Stable Aberrations by Multiple Fluorescence In Situ Hybridization (mFISH) and Spectral Karyotyping (SKY) in Irradiated Mice

9.8K Views

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10:14 min

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January 11th, 2017

DOI :

10.3791/55162-v

January 11th, 2017

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Rupak Pathak¹, Igor Koturbash², Martin Hauer-Jensen¹^,³

¹Division of Radiation Health, Department of Pharmaceutical Sciences, College of Pharmacy, University of Arkansas for Medical Sciences, ²Department of Environmental Health, Fay W. Boozman School of Public Health, University of Arkansas for Medical Sciences, ³Surgical Service, Central Arkansas Veterans Healthcare System

Summary

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Keywords MFISH

Spectral Karyotyping

Inter chromosomal Stable Aberrations

Radiation Biology

Bone Marrow Cells

Metaphase Cell Spreads

Mouse Chromosome Painting

Chapters in this video

0:05

Title

0:53

Isolation of Bone Marrow Mononuclear Cells by Density Gradient Centrifugation and Preparation of Metaphase Cell Spreads

3:04

Mouse Chromosome Painting by mFISH

6:07

Spectral Karyotyping of Mouse Chromosomes

7:45

Results: Inter-chromosomal Stable Aberration Analysis by Multiple Fluorescence In Situ Hybridization and Spectral Karyotyping

9:02

Conclusion

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