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Rapid Isolation of the Mitoribosome from HEK Cells

DOI :

10.3791/57877-v

9:33 min

October 4th, 2018

October 4th, 2018

10,098 Views

1Science for Life Laboratory, Department of Biochemistry and Biophysics, Stockholm University

Mitochondria have specialized ribosomes that diverged from their bacterial and cytoplasmic counterparts. Here we show how mitoribosomes can be obtained from their native compartment in HEK cells. The method involves isolation of mitochondria from suspension cells and consequent purification of mitoribosomes.

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Mitoribosome Isolation

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