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Bidirectional Retroviral Integration Site PCR Methodology and Quantitative Data Analysis Workflow

10.7K Views

12:53 min

June 14th, 2017

DOI :

10.3791/55812-v

June 14th, 2017


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Keywords Bidirectional Retroviral Integration Site PCR

Chapters in this video

0:05

Title

1:11

LTR-specific Biotin-primer Extension

2:53

Rsal and CviQI Digestion and Blunt-ending

4:12

Straptavidin Bead Binding

5:31

Linker Ligation

6:39

Pre-amplification of Both Left and Right Junction DNA

8:15

Left- and Right-junction-specific Amplifications

10:23

Results: The IS Bidirectional Assay Improves Detection Rates and Sequence Quantification Accuracy

12:09

Conclusion

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