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Detection of Low Copy Number Integrated Viral DNA Formed by In Vitro Hepatitis B Infection

DOI :

10.3791/58202-v

11:14 min

November 7th, 2018

November 7th, 2018

17,502 Views

1Department of Infectious Diseases, Molecular Virology, Heidelberg University Hospital, 2Gastroenterology and Liver Research Laboratory, Ingham Institute, 3German Center for Infection Research (DZIF)

We describe here the in vitro generation of HBV DNA via a Hepatitis B virus infection system and the highly sensitive detection of its (1–2 copies) integration using inverse nested PCR.

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Low Copy Number

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