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Measurement of Protein Turnover Rates in Senescent and Non-Dividing Cultured Cells with Metabolic Labeling and Mass Spectrometry

DOI :

10.3791/63835-v

8:52 min

April 6th, 2022

April 6th, 2022

2,784 Views

1Laboratory of Genetics and Genomics, National Institute on Aging Intramural Research Program, National Institutes of Health, 2Translational Gerontology Branch, National Institute on Aging Intramural Research Program, National Institutes of Health

This protocol describes the workflow for metabolic labeling of senescent and non-dividing cells with pulsed SILAC, untargeted mass spectrometry analysis, and a streamlined calculation of protein half-lives.

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Protein Turnover Rates

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