Isolation of Total RNA from Phenol-guanidine Isothiocyanate Solution Lysate
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Analysis of Transcript Abundance
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Results: Clear Contrast in Transcript Decay Between Proliferating and Quiescent Fibroblasts
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Conclusion
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The overall goal of this procedure is to monitor transcript decay rates in proliferating and quiescent fibroblasts. This method can help answer key questions in the molecular biology field such as whether changes in gene expression result from cha
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We describe a protocol for generating proliferating and quiescent primary human dermal fibroblasts, monitoring transcript decay rates, and identifying differentially decaying genes.