JoVE Logo
Faculty Resource Center

Sign In

Detection of Protein Aggregation using Fluorescence Correlation Spectroscopy

DOI :

10.3791/62576-v

April 25th, 2021

April 25th, 2021

4,939 Views

1Laboratory for Molecular Cell Dynamics, Faculty of Advanced Life Science, Hokkaido University

We here introduce a procedure to measure protein oligomers and aggregation in cell lysate and live cells using fluorescence correlation spectroscopy.

-- Views

Related Videos

article

An Antimicrobial Fabric Using Nano-Herbal Encapsulation of Essential Oils

article

Direct Synthesis of EM-Visible Gold Nanoparticles in Cells for Protein Localization Analysis with Well-Preserved Ultrastructure

article

Fluorescence-Based Quantification of Mitochondrial Membrane Potential and Superoxide Levels Using Live Imaging in HeLa Cells

article

Detection of Mitophagy in Caenorhabditis elegans and Mammalian Cells Using Organelle-Specific Dyes

article

Two-Step Tag-Free Isolation of Mitochondria for Improved Protein Discovery and Quantification

article

Author Spotlight: Exploring Cellular Zinc Regulation Through ZnT1 Functionality

article

Imaging ATG9A, a Multi-Spanning Membrane Protein

article

Alignment of Synchronized Time-Series Data Using the Characterizing Loss of Cell Cycle Synchrony Model for Cross-Experiment Comparisons

article

Author Spotlight: A Novel Approach for Embedding Cell-Free Protein Synthesis Reactions in Hydrogels

article

Author Spotlight: The Production of Recombinant Proteins

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved