JoVE Logo
Faculty Resource Center

Sign In

Detection of Protein Aggregation using Fluorescence Correlation Spectroscopy

DOI :

10.3791/62576-v

April 25th, 2021

April 25th, 2021

5,017 Views

1Laboratory for Molecular Cell Dynamics, Faculty of Advanced Life Science, Hokkaido University

We here introduce a procedure to measure protein oligomers and aggregation in cell lysate and live cells using fluorescence correlation spectroscopy.

-- Views

Related Videos

article

Visualizing Single Molecular Complexes In Vivo Using Advanced Fluorescence Microscopy

article

Using the GELFREE 8100 Fractionation System for Molecular Weight-Based Fractionation with Liquid Phase Recovery

article

Time-lapse Imaging of Mitosis After siRNA Transfection

article

Monitoring of Ubiquitin-proteasome Activity in Living Cells Using a Degron (dgn)-destabilized Green Fluorescent Protein (GFP)-based Reporter Protein

article

Detection of Rare Genomic Variants from Pooled Sequencing Using SPLINTER

article

Nano-fEM: Protein Localization Using Photo-activated Localization Microscopy and Electron Microscopy

article

Cell Specific Analysis of Arabidopsis Leaves Using Fluorescence Activated Cell Sorting

article

Quantitative Analysis of Autophagy using Advanced 3D Fluorescence Microscopy

article

4D Imaging of Protein Aggregation in Live Cells

article

Visualizing mRNA: Fluorescence In Situ Hybridization

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved