Measurement of Luciferase Reporter Signals, which Correspond to γ-Secretase Cleavage of APP-C99 (CG cells) or NΔE (NG cells)
3:42
Quantification of γ-Secretase Activity
4:40
Results: Treatments with CL-387, 785 can Differentially Promote the Processing of APP-C99 by γ-Secretase without Affecting Notch Cleavage
6:05
Conclusion
Transcript
The overall goal of this quantitative cell-based reporter gene assay for gamma-secretase is to examine the differential effect of putative gamma-secretase modulators on gamma-secretase-mediated cleavage. This method can help to answer the key ques
Sign in or start your free trial to access this content
We have successfully generated two substrate-specific γ-secretase assays. Both cell-based assays presented here are designed to quantify γ-secretase enzymatic activities via the output of firefly luciferase reporters.