JoVE Logo
Faculty Resource Center

Sign In

Preparation and Observation of Thick Biological Samples by Scanning Transmission Electron Tomography

DOI :

10.3791/55215-v

March 12th, 2017

March 12th, 2017

9,051 Views

1Institut Curie, INSERM U1196, Campus Universitaire d'Orsay, 2Institut Pasteur, Trypanosome Cell Biology Unit, Department of Parasites & Insect Vectors, INSERM U1201

This report describes a sample preparation protocol and specific imaging conditions for performing scanning transmission electron tomography of thick biological specimens.

Tags

Scanning Transmission Electron Tomography

-- Views

Related Videos

article

Visualization of ATP Synthase Dimers in Mitochondria by Electron Cryo-tomography

article

Flat Mount Preparation for Observation and Analysis of Zebrafish Embryo Specimens Stained by Whole Mount In situ Hybridization

article

Studying the Supramolecular Organization of Photosynthetic Membranes within Freeze-fractured Leaf Tissues by Cryo-scanning Electron Microscopy

article

Scanning Electron Microscopy (SEM) Protocols for Problematic Plant, Oomycete, and Fungal Samples

article

Sample Preparation and Imaging of Exosomes by Transmission Electron Microscopy

article

Visualization of DNA Compaction in Cyanobacteria by High-voltage Cryo-electron Tomography

article

Miniaturized Sample Preparation for Transmission Electron Microscopy

article

Biological Sample Preparation by High-pressure Freezing, Microwave-assisted Contrast Enhancement, and Minimal Resin Embedding for Volume Imaging

article

Array Tomography Workflow for the Targeted Acquisition of Volume Information using Scanning Electron Microscopy

article

Serial Block-Face Scanning Electron Microscopy (SBF-SEM) of Biological Tissue Samples

JoVE Logo

Privacy

Terms of Use

Policies

Research

Education

ABOUT JoVE

Copyright © 2024 MyJoVE Corporation. All rights reserved