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Dissection and Explant Culture of Murine Allantois for the In Vitro Analysis of Allantoic Attachment

DOI :

10.3791/56712-v

January 13th, 2018

January 13th, 2018

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1Institute of Pharmacology and Toxicology, University of Würzburg, 2Rudolf Virchow Center for Experimental Biomedicine, University of Würzburg

We describe an in vitro assay to model chorioallantoic attachment, the first step in placenta formation. The protocol demonstrates the dissection and explant culture of murine allantoides on immobilized α4β1 integrin. Allantois attachment is evaluated microscopically at pre-determined time points.

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