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Microdissection and Dissociation of the Murine Oviduct: Individual Segment Identification and Single Cell Isolation

DOI :

10.3791/63168-v

November 4th, 2021

November 4th, 2021

2,964 Views

1Division of Biomedical Sciences, School of Medicine, University of California, Riverside, 2Microscopy and Imaging Core Facility, University of California, Riverside

A method for microdissection of the mouse oviduct that allows collection of the individual segments while maintaining RNA integrity is presented. In addition, non-enzymatic oviductal cell dissociation procedure is described. The methods are appropriate for subsequent gene and protein analysis of the functionally different oviductal segments and dissociated oviductal cells.

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