GFP-Fusion Protein Expression and Anti-IgG Antibody Glass Coverslip Coating
3:14
Cell Spreading, Fixing, and Immunostaining
5:44
Stimulated Emission Depletion Microscope Imaging
8:02
Results: Representative STED Imaging
9:50
Conclusion
필기록
The overall goal of this protocol is to stain actin and microtubule cytoskeletons at the B-cell immune synapse for stimulated emission depletion, or STED microscopy, and imaging their structure, organization in spatial relationships. This method c
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We present a protocol for using STED microscopy to simultaneously image actin structures, microtubules, and microtubule plus-end binding proteins in B cells that have spread on coverslips coated with antibodies to the B-cell receptor, a model for the initial phase of immune synapse formation.