Applying Live Cell Imaging and Cryo-Electron Tomography to Resolve Spatiotemporal Features of the Legionella pneumophila Dot/Icm Secretion System
6.9K Views
•
09:12 min
•
March 10th, 2020
DOI :
March 10th, 2020
•Chapters in this video
0:04
Introduction
0:55
Live Cell Imaging of L. pneumophila with Fluorescently Tagged Dot/Icm Components
2:47
Quantification of Polar Localization and Dynamics of Dot/Icm Components
5:36
Detection of sfGFP Mass Density with Cryo-ET
6:23
Results: Imaging Dot/Icm Secretion Systems in Intact Bacteria
8:17
Conclusion
Related Videos
07:31
Live Cell Imaging of Bacillus subtilis and Streptococcus pneumoniae using Automated Time-lapse Microscopy
42.3K Views
09:25
Purification of Pathogen Vacuoles from Legionella-infected Phagocytes
12.4K Views
14:29
Visualization of Bacterial Toxin Induced Responses Using Live Cell Fluorescence Microscopy
11.7K Views
12:30
Use of Galleria mellonella as a Model Organism to Study Legionella pneumophila Infection
40.2K Views
14:05
Imaging InlC Secretion to Investigate Cellular Infection by the Bacterial Pathogen Listeria monocytogenes
9.8K Views
09:09
A Label-free Technique for the Spatio-temporal Imaging of Single Cell Secretions
8.5K Views
11:26
Visualizing Single Molecular Complexes In Vivo Using Advanced Fluorescence Microscopy
9.3K Views
11:33
Using Tomoauto: A Protocol for High-throughput Automated Cryo-electron Tomography
10.9K Views
14:23
Electron Cryotomography of Bacterial Cells
25.3K Views
08:44
Visualization of Twitching Motility and Characterization of the Role of the PilG in Xylella fastidiosa
10.7K Views
Copyright © 2025 MyJoVE Corporation. All rights reserved